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We further removed low complexity sequences using mDust [ 38].
We have not removed low complexity regions because SLiMs frequently occur within them.
Additionally, we include a sequence complexity filter based on entropy, which removed low complexity reads with entropy less or equal to 50.
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It was not necessary to mask the P. vivax sequence as removing low complexity regions in P. falciparum sequence will prevent P. falciparum low complexity regions from aligning with orthologous P. vivax low complexity regions.
In this step, we also remove low complexity reads that likely result from process artifacts [ 28].
Sequences were cleaned to remove low complexity regions, short length (<100 bp), and vector and adaptor sequence using seqclean [ 75].
Transposable element reference used for comparison was first filtered to remove low complexity and satellite repeats, to focus on the major transposable elements sub families.
The output sequences were then filtered by Seqclean (http://compbio.dfci.harvard.edu/tgi/software/) to remove low complexity sequences and vector contaminations.
The results were filtered by removing low complexity sequences and sequences shorter than 100 nt, and retaining only repeats having at least 10 matches when mapped onto the original 454 set using RepeatMasker [ 62].
After adapter trimming, we used SeqClean for identifying and removing low complexity regions, overly short reads (shorter than 60 bp), remains of polyA tails, and reads with high similarity to mammalian repetitive sequences in RepBase ver. 14.09.
To exclude adenosine-rich reads mapping with low confidence to genome-encoded oligo(A) tracts, we removed low-complexity reads (which we defined as reads with genome-encoded portions containing fewer than 8 nonmodal nucleotides).
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