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IL-3 was removed from the medium on day 4 and SCF on day 6.
Any additional cell debris was removed from the medium by filtration through 0.22-μm PES membrane filters (Thermo Fisher Scientific).
There were significant differences in growth (p = 0.005) when C. parriaudii was cultivated in a single and multi-N source medium: NH4+ was preferentially removed from the medium, whereas urea was typically removed secondarily.
The drug was then removed from the medium, cells were continued to grow in culture and the amount of FITC fluorescence retained per cell was measured using a fluorescence-activated cell flow analyzer (FACScan, Becton-Dickinson, San Jose, CA, USA) at various intervals.
At predetermined intervals, the hydrogel was removed from the medium, gently blotted and weighed.
HPLC analysis demonstrated that TNT was efficiently removed from the medium during yeast growth (Figure 1A).
Twenty-one-day-old seedlings of the wild type and ZmNrt2.1-expressing plants grown on MS medium were removed from the medium, gently rinsed with water and incubated with gentle shaking for 1 h in water, 5 mM LaCl3, 10 μM U73122 or 10 μM U73343, followed by incubation for 1 h in either 5 mM KNO3 or 5 mM KCl, according to Riveras et al. (2015).
The next day, cells were removed from the medium by centrifugation.
All larvae were removed from the medium, rinsed in distilled water, and blotted dry before use in experiments.
Forced expression of UCP1 did not significantly depress the ATP level relative to pRev control unless glucose was removed from the medium.
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The 5-Aza-CdR was removed from the culture medium at 24 h, and regular medium was used thereafter.
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