When the SEM chamber was sealed and moisture was vacuumed from the testing chamber, cracks formed in the plastic because the moisture was being removed from the plastic.
At the two-leaf stage, seedlings were removed from the plastic plates, and the roots were rinsed with distilled water.
After 48 h of incubation, the samples were removed from the plastic wells, washed three times with PBS, fixed with cold methanol, and dried before microscopic examination.
The posts involved with CR were removed from the plastic matrix and stored in distilled water for 24 h in an oven (ECB 3; Odontobrás, Ribeirão Preto, SP, Brazil) at 37 °C.
After 24, 48, and 72 h of incubation, the samples prepared as described above were removed from the plastic wells, washed three times with PBS, fixed with cold methanol, and dried before microscopic examination.
After 48 and 72 h of incubation, the samples were removed from the plastic wells, washed three times with PBS, fixed with cold methanol, and dried before microscopic examination.
In order to minimize the contamination from any fortuitous substances such as dust particles and fibers, crystallization plates were removed from their plastic covers just prior to setting up the experiments and sealed with sealing tape (Qiagen Inc ,California, U.S.A).
Before replanting, the saplings were carefully removed from the plastic bags to avoid damage to the root system; loose soil, i.e., not adhering to the roots, was discarded.
