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Asphaltenes were removed from approximately 20 50 mg oils and bitumen by precipitation using 50 mL of n-hexane and then fractionated by liquid chromatography using alumina/silica gel columns into saturated and aromatic hydrocarbons using 30 mL n-hexane and 20 mL dichloromethane: n-hexane (2 1, v:v) as respective eluents (Fang et al. 2015).
The resulting fluorescence was expressed as relative ROS production according to this equation:
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Since the immuno-depletion column employed removes from approximately 65%to85%5% of the total plasma protein (Agilent, personal communication; [ 20]), the amount of protein loaded in the 09_OGE_6_depl_sample was correspondingly reduced to 650 ug to compensate for the loading of the non-depleted 1800 ug from 08_OGE.
We also removed from consideration approximately 4,000 SNPs due to a high percentage of missing entries (over 10%).
When excessively noisy data was removed from analysis (approximately 19% of recordings), the sensitivity and specificity increased to 89.4% and 91.5% respectively.
Cages contained a wicked bottle of 10% sugar solution, which was removed from cages approximately 16 h prior to the first opportunity to blood-feed and returned after the second opportunity had finished.
As much seawater as possible was then removed from samples and approximately 2 ml of 95% ethanol was added to each vial.
Growth medium was removed from cells at approximately 90% confluence, and sterile PBS was used to wash the cells once.
In this manner, 87 contigs were removed from the database (approximately 200 Kb in total), resulting in a genome of 2.87 Mb (GC content 38.4%).
Based on older studies, in patients with renal failure undergoing peritoneal dialysis, approximately 1 mg/h of colistin is removed from the patient and approximately 16% of the total colistin dose is removed during a 2-h peritoneal dialysis session.
The specimens were sectioned knee joints and had been removed from the cadaver at approximately mid-femur and mid-tibia.
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