Whole spices are easy to remove from a dish prior to serving and unless the recipe says otherwise, usually should be removed.
To study shoot emergence, a rootling was removed from the dish and planted in a 7-cm-deep plastic container, filled with 2 3 cm of moist sand, and covered with plastic film; only the root and lower portion of the seed were covered with sand.
The cell sheet was then removed from the dish using a cell shifter (CellSeed).
After treatment, cells were removed from the dish, resuspended in buffer A (20 m M Hepes-KOH, pH 7.5, 10 m M KCl, 1.5 m M MgCl2, 1 m M EDTA, 1 m M EGTA, 1 m M DTT, 250 m M sucrose, and protease inhibitors), and homogenised using a teflon homogeniser.
After apoptosis induction or mtDNA copy number regulation for a certain time, the culture medium was removed from the dish, and the samples were washed with PBS.
100 µl of this culture was plated onto the Petri dish containing the embedded slide and allowed to dry for 30 min. The slide was removed from the Petri dish, a cover slip placed on top and the excess solid medium trimmed from around the edges of the cover slip.
The PDMS is removed from Petri dish and the 20 1 strips are carefully removed using forceps and a razor blade so as to not damage the 5 1 mold.
Seeds were checked for an emerged radicle fortnightly, and if present, counted and removed from the dish.
The sugarcane samples were then removed from the Petri dish using a pipette with a wide-orifice tip and placed onto optically transparent calcium fluoride windows (International Crystal Labs, Garfield, NJ, USA).
The EDTA was then removed from the dish and replaced with 3.6 ml of calcium/magnesium-free PBS.
