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Several tissues were removed for detection of metastases, which were primarily observed in the lungs.
After sacrification of animals infected with 20 μl of the 2% inoculum the ipsilateral and contralateral Ln. popliteus were removed for detection of PrPTSE by Western blotting (n = 2 for the following time points each: 2 days post infection (dpi), 42-, 70-, 80-, 90- and 100 dpi).
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Then, 200 μl of each supernatant aliquot was removed for Yersinia detection by rtPCR and the rest of the suspension was stored at -20°C for microbiological studies.
The supernatant was removed for cortisol level detection.
Following euthanization using CO2, mouse hind limbs, lungs, livers, kidneys and other tissues were removed for separate imaging, and detection of metastases.
Of 486,428 probes present on the array, 11,648 X and Y chromosome probes were removed; 1,927 were removed for failing to meet the detection P-value cut-off (P = 0.05) and a further 43,097 were removed due to probe cross-hybridization.
Livers were quickly removed for the NT and cystathionine β-synthase detection.
In fact, we invested over $60 million last year alone, and, in the last 6 months of 2010, more than 95% of accounts removed for counterfeits came down based on our own detection efforts.
(Discussion removed for confidentiality).
Video surveillance techniques for abandoned and removed object detection have received great attention in the last few years.
Another three individuals were removed based on detection p-values (> 0.01 for > 1% of probes) and 3,027 of 27,578 probes were excluded based on detection values (p>0.01 for > 1% of the samples).
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CEO of Professional Science Editing for Scientists @ prosciediting.com