Thoracic aorta was removed, flushed with cold isotonic saline, carefully cleaned of perivascular adipose tissue and flash frozen in liquid nitrogen before storage at -80°C until analysis.
Forty-eight hours later, colons were removed, flushed with PBS, fixed in 10% neutral buffered formalin overnight, embedded in paraffin and sectioned at 5 μm thickness.
After animals were sacrificed, colons were quickly removed, flushed with saline solution, and longitudinally slit-opened from cecum to anus, placed on a paper towel, and fixed in 10% buffered formalin for 24 h.
Randomly selected hearts from control and cardiomyopathic turkey poults were rapidly removed, flushed with PBS and infused with a tissue embedding medium (Tissue-Tek, OCT Compound, Miles Inc., Elkhart, IN) and frozen in liquid nitrogen.
+/- = PCR product present/absent respectively (see Methods) Mice were killed by CO2 asphyxiation and the small intestine removed, flushed with phosphate buffered saline, opened lengthwise, and mounted en face.
Colons were removed, flushed with PBS, fixed in 10% neutral buffered formalin overnight, embedded in paraffin, sectioned at 5 μm and stained with hematoxylin and eosin for histopathological evaluation of inflammation and neoplasia.
Because of the patchy nature of colitis in IL10−/− mice whole colons were removed, flushed with PBS, and a quarter of the tissue was cut longitudinally and resuspended in tissue culture plates (Falcon 3046; Becton Dickinson Labware, Lincoln Park, New Jersey, USA) in RPMI 1640 supplemented with 10% fetal calf serum, penicillin (100 U/ml), streptomycin (100 U/ml).
Flush with rubbing alcohol first, then, when all the substance has been removed, proceed flushing with water, and the rest of the above steps.
The extraneous matrix interference was removed by flushing the extraction column with a gradient elution.
After 15 min CFA or IS was removed by flushing with saline.
The excess of suspension in the pores of the foam was removed by flushing with an air flow.
