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Initially, dorsal hairs were removed externally by shaving (∼2×6 cm) using an electric trimmer (Wahl Stylique Designer/Liner pet trimmer; 919179; Petco, San Diego, CA, USA) revealing pink skin.
This approach takes advantage of the ability to see new black hair growth easily in the pink unpigmented skin, once the fully formed resting (telogen) hair is removed externally with clippers (26).
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Uptake was analysed after 60 min incubation of CHO-K1 cells with different concentrations of peptides at 37°C and stringent washing with Heparin to remove externally bound peptide as described previously [ 31].
Cells from cultures were harvested and washed twice with wash buffer (0.5 g l−1 Na2PO4, 1.74 g l−1 KH2PO4 and 1.06 g l−1 NH4Cl pH 6.9) containing 500 μM EDDS (Sigma) to remove externally bound or free remaining zinc.
Alleles that reduce the fitness in a given genetic background can be removed by externally (e.g. ecological) caused natural selection.
It will maintain this deformed shape after subsequently lowering the temperature to TLremoving the externally mechanical load.
Washing hair with soap and hot water should remove most externally bound contaminants and, theoretically, may allow for the determination of internally bound analytes.
For isolation of mitochondria, 40 g of leaf material was sampled 24 h after spraying and leaves were extensively washed for at least 5 min at 4°C in 5 L distilled water with slow agitation to remove any externally persisting detergent or inhibitor.
The model, a wrasse (Labroides dimidiatus) of the Indo-Pacific Ocean, is known as a cleaner fish because it removes and eats externally attached parasites and, occasionally, damaged skin fragments from other marine fish.
Finally, inappropriate content on company-owned ESSPs, either internal-only or externally visible, can always be removed if necessary.
When the soft parts of the animal are removed from the shell, several organs are distinguishable externally, including the kidney, the nephiridial gland, the pericardium, the genital glands, stomach, style sac and the digestive gland.
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