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The mice were sacrificed at 1, 6, 24, and 72 h, and the organs (spleen, kidney, liver, thymus, and tumor) were immediately removed, embedded in optimal cutting temperature (OCT) compound and frozen (−78.5 °C).
Inflated lungs were then removed, embedded in Tissue-Tek OCT compound and stored at −80°C.
Each heart was removed, embedded in O.C.T.™ Compound (Tissue-Tek®; Zoeterwoude, Niederlande) and snap-frozen in liquid nitrogen.
After anesthesia, animals were perfusion-fixed with 4% paraformaldehyde in 0.1 M phosphate buffer (pH 7.2) (Sigma), after which brains were removed, embedded in paraffin and sectioned.
Ovaries were removed, embedded in OCT medium (Tissue-Tek), snap frozen in liquid N2 and 10 µm sections cut with a cryostat.
The injured as well as the contralateral carotid arteries were then removed, embedded in OCT TissueTec (Sakura Finetec, Netherlands) and frozen for histology.
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Powerful, yet gentle suction removes embedded wax without injury.
Related tasks like removing embedded IDAT from image data or free text forms, or preventing re-identification of patients through quasi-identifying attributes in the MDAT are research areas of their own and are not within the scope of this article.
It is probably best to see a doctor before considering trying to use a needle to remove embedded hairs.
Optimizer saves space by removing embedded and duplicate fonts, compressing images, and removing items from the file that are no longer needed.
The ovaries were removed and embedded in O.C.T. embedding matrix (CML, ref KMA-0100-00A, frozen), frozen in liquid nitrogen and stored at -80°C until use.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com