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He co-sponsored legislation in the weeks after Bush's decision, which stated that no "historic flag commemorating or memorializing the American Revolutionary War, the War of 1812, the Mexican War, the Civil War... displayed on public property of the state or any of its political subdivisions may be relocated, removed, disturbed or altered".
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After incubation, the supernatant was removed without disturbing the cells and 50 µL DMSO was added to each well to dissolve the formazan crystals.
After clot formation, the serum was completely removed without disturbing the clot, and each tube having the clot was again weighed to determine the clot weight (Clot weight = Weight of clot containing tube − Weight of tube alone).
"Buying a new toaster's not going to do you much good, nor will buying anything that can be removed without disturbing the house but let's say you remodeled the bathrooms and added a new fireplace; those costs can be added to your original cost basis," Ondik says.
The supernatant PBS was completely removed without disturbing the pellet and a ∼10 µl fraction of the pellet was loaded onto a microscope slide for direct LAESI analysis.
The tail domains (Vt, MVt or MVt-ΔH1) were incubated with or without F-actin at room temperature for 10 min and centrifuged at low speed (6,000 r.p.m.; Eppendroff table top centrifuge) for 10 min. The supernatant was removed without disturbing the pellet and stored separately while the pellet was washed gently by layering with 100 µl of PBS and removing the excess buffer.
The supernatant was removed without disturbing the cell button.
The supernatant was removed without disturbing the pellet and the cells were rinsed.
After a 2 min centrifugation at 14,000 g, the MeOH was removed without disturbing the pellet.
Gut and fat body were removed without disturbing the inner epithelial layer.
The supernatants are carefully removed without disturbing the sediment and the pellet is dissolved in phosphate buffer (pH 7.5).
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