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After 3 hours transfection media is removed, cells are washed, and fresh growth media is added.
The supernatant is removed, cells are resuspended in LDF with fetal bovine serum, and spun again at 500 g for 5 minutes at 4°C.
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Every 2 3 days, medium was removed, cells were washed with PBS (Gibco) and fresh medium was added.
After the treatment, medium was removed, cells were washed three times with PBS and fresh medium was added.
Every 3 days, non-adherent cells were removed, cells were washed, and culture medium was replaced.
After incubation, medium was removed, cells were washed with binding buffer and radioactivity counted.
After this time treatment media was removed, cells were washed twice and fresh media only was added to each well.
Twenty-four hours after the final treatment, the media was removed, cells were washed with PBS and fresh media was added.
After 24 h medium was removed, cells were washed twice with PBS, and incubated with FCS-free medium.
Inoculum was removed, cells were washed and then subjected to infection by an adenovirus construct encoding KSHV RTA (Ad-RTA).
After 1 h of labelling, medium was removed, cells were washed three times with ice cold PBS.
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CEO of Professional Science Editing for Scientists @ prosciediting.com