Although spreading cancer cells might be removed, cells that are fighting the cancer also are removed.
Every 2 3 days, medium was removed, cells were washed with PBS (Gibco) and fresh medium was added.
Medium was removed, cells washed twice with 500 µL of PBS, counted and lysed using 500 µL of SDS 2% during 1 h.
We removed cells with (1) <500 detected (transcript count > 0) genes, and (2) >10% of transcript counts mapped to mitochondrial genes.
In Dr. Black's study, the investigators removed cells from the bone marrow of rats -- and of humans -- that normally would develop into tendon, cartilage, bone, muscle and fat, and grew them in the laboratory in shallow dishes.
Decellularization removed cells but preserved the 3-dimensional cardiac macro and microstructure and the native vascular network in a perfusable state.
The drug was then removed, cells were continued to grow in culture and the amount of fluorescence retained per cell was measured using a fluorescence-activated cell flow analyzer.
After the treatment, medium was removed, cells were washed three times with PBS and fresh medium was added.
Both sheep IgG and hemoglobin were demonstrated immunocytochemically in vacuoles most of which could also be shown to contain cathepsin D. When the antiserum was removed, cells regained their normal morphology, and digestion of endocytosed proteins returned to normal.
A group of physicians in Poland removed cells from a quadriplegic's olfactory nerve (the only nerves that regenerate) and injected into his spinal cord.
After 3 hours transfection media is removed, cells are washed, and fresh growth media is added.
