Sentence examples for removed cell layers from inspiring English sources

Prior to measurement of ATP levels, culture medium was removed, cell layers were washed and cells were incubated with serum-free DMEM (1 ml/well) for 1 h.

When applied to our study, this model suggests that the induction of KRT6B and KRT16 would reflect a transient change in keratinocyte cytoskeleton in order to adapt to both the hyperproliferative stimulus triggered by TS and the need for sufficiently resilient cells to replace removed cells in suprabasal layers.

Flasks were cooled on ice, and culture medium removed and cell layers washed with phosphate buffered saline.

After incubation, media were removed and cell layers were extracted according to the manufacturer's protocol and analyzed for collagen II levels using an ELISA kit (MD Biosciences Inc., St Paul, MN, USA).

After incubation the culture media were removed, the cell layers were washed with PBS and submitted to the action of lysis buffer for determination of ORP150/GRP170 expression and protein assay.

After this time the medium was removed and the cell layers ware washed with 2 mL of PBS.

The medium was removed, and the cell layers were rinsed three times with PBS and fixed with 95% ethanol at room temperature for 15 min.

At days 2 and 6 of culture, the supernatant of each well was removed and the cell layers were washed twice with PBS.

After 24 hours, the apical medium was removed from the confluent cell layers and the direct exposure at the air-liquid interface with the CULTEX RFS was started.

Cells are chilled, media is removed, and the cell layer is rinsed three times with cold PBS.

To do this, the medium was removed and the cell layer washed 3 times with PBS without calcium and magnesium.