Supernatants were harvested from cell cultures treated with SIL, silibinin, or relevant solvent controls (PBS or DMSO), and filtered through 10,000 molecular weight cutoff filters (UFC501096 Amicon Ultra-0.5, Ultracel-10 Membrane) to remove carry over of SIL and silibinin.
At 3 h post-infection, the conditioned media was collected from experiment and control samples and subjected to two sequential centrifugation steps at 1,200 rpm for 2 min to remove carry over non-adherent host cells, followed by 4,000 rpm for 10 min to pellet extracellular bacteria.
In an emergency, the incubator's bassinet can be removed and carried to another part of the building or even to another hospital.
If the victim recovered, the shrines were removed and carried away in a special paper chair or boat for burning.
(B) Probe trials with the platform removed were carried out on day 5. Figure S2.
During the soaking process, about 46% of the soluble solids are removed and carried in the soak water.
If a complex contains more than one symmetric unit, redundant units are removed, as carried out by Ponomarenko and Bourne (2007).
It usually has a base that is strapped into the back seat of a car, and a snap-in seat that can be removed and carried.
Woland has a giant talking cat as an assistant, and carries out a stage trick in which a man's head is removed and carries on talking, which is likely not the trick of a mere illusionist.
It'll mean better and lighter trains, and a faster service to and from St Pancras," said the transport secretary.. Carrying the red cabinet boxes as secretary of state is a long way removed from carrying coal when he worked underground at Littleton Colliery near Cannock.
He said he made sure the bodies were removed quickly, carrying away one himself.
