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Contaminating genomic DNA was removed by two consecutive incubations using the DNA-free DNase Treatment and Removal kit (Applied Biosystems).
Excess adaptors were removed by two volumes of AMPureXP beads as manufacturer's instruction.
Excess antibody was removed by two 30 min washes in Tween-PBS buffer, and the membrane was incubated for 1 h with an anti-rat IgG solution.
Unbound virus was removed by two washes with cold media.
This vector is PCA transformed, and the noise is removed by two shrinkage stages.
MB is removed by two ways biodegradation and adsorption on bacterial surface.
Surface-bound activity was removed by two 5-min acid washes (50 mM glycin-HCl, 100 mM NaCl, pH 2.8).
Unbound radioligand was removed by two washes with 250 μL TRIS buffered saline, and the remaining radioactivity on the filter was measured in the gamma counter.
In "The Build," a sculpture constructed of blocks is missing a piece of his knee, which has been removed by two smaller figures at his feet.
"Some 28,000 litres of waste matter has been removed by two suction tank lorries, and is currently being stored pending disposal," Hee-Loy Sian, executive council in charge of the environment, told the Straits Times.
Excess unbound free dye was removed by two consecutive size-exclusion columns (Bio-Rad DG).
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CEO of Professional Science Editing for Scientists @ prosciediting.com