Exact(2)
Before pre-clearing, 50 μl of the cleared lysate was removed as input for total RNA profiling.
The sheared chromatin was resuspended in dilution buffer and 1% of the chromatin was removed as input, followed by immunoprecipitation using protein G magnetic beads with 2 μg of either anti-WT1 (C-19) antibody (Santa Cruz Biotechnology Inc, Santa Cruz, CA, USA,) or normal rabbit IgG (Cell Signalling Technology Inc, Danvers, MA, USA) at 4°C overnight with rotation.
Similar(6)
For the third paradigm (lateralised sensory input), 200 bees were marked at emergence and had both, one, or no antennae removed as follows.
One tenth of the lysate was removed as an input sample.
Before immunoprecipitation, 120th of the sample was removed as the input sample.
Remove as soon as it's done.
Remove as much air as possible.
50 µL of sample was removed to serve as input control, whereas 50 µg of protein was used for each immunoprecipitation, including IgG and serum controls.
Related(1)
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