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Briefly, the encoded microparticles with a polycation upper layer were additionally coated with a polyanion polyacrylic acid (PAA) with Mw ≈ 15,000 Da (Sigma-Aldrich, USA), and the core was removed as described above.
(c) Absolute value of the response in control and muscarine conditions, baseline removed as described in (b), averaged over 7 cells and normalized by Nq (N number of synapses, q quantal amplitude of single synapse) to have units of "probability of release", Pr.
Vector sequences were removed as described above.
Flagellins were purified and contaminating lipopolysaccharide (LPS) was removed as described in [27].
At day 28 animals were anaesthetized by isoflurane before the pancreas was removed as described above.
After the peritoneal wash, rodents were also exsanguinated and kidneys were removed as described above.
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From these data, six samples with low inter-array correlation were removed as outliers (as described in [ 5]).
The pixel sample registers determine the real sampled pixels when set to 1, while configured to 0 produces a false value to be removed as previously described.
Chromatograms of nearly full-length 16S rRNA gene sequences were assembled, trimmed, quality-checked, and chimeric sequences removed as previously described [18].
Next, the Fmoc-protecting group was removed as previously described.
Low quality reads were removed as previously described [ 21].
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