In this case, non-replicating mtDNAs are removed and replicating mtDNAs are capable of fast enough replication to survive the transient drop in copy number.
MutL also activates UvrD in bacteria to unwind the DNA (Yamaguchi et al., 1998), after which the new DNA strand can be removed and re-replicated (Kunkel and Erie, 2005).
In TNT v1.1 [ 35] node support was assessed using jackknife resampling with 36% of characters removed and 1000 replicates, using a heuristic search with tree bisection-reconnection (TBR) branch swapping and 1000 random addition sequence replicates.
Virus-containing supernatant was removed, and virus was allowed to replicate for 4 h.
At set intervals replicate wells were removed and dual luciferase assays were performed on the cell lysates.
For genes with p<0.05, the average ratio was used as the final -fold change; for genes with p>0.05, the inconsistent replicates (with distance to the median of replicate ratios >0.8) were removed, and only the values from genes with at least two replicates in the same direction of regulation were further averaged.
Concurrent to removing test pegs for the indirect qualitative outgrowth test, replicate sample pegs were removed and directly examined for viability by the quantitative viability counting method and direct qualitative outgrowth test for comparison.
A shows the outcome when all individual data sets were considered unique, B shows the outcome when replicate experiments were removed and C shows the outcome when data sets overlapping between the training and test sets were removed from the test set.
Certain parameters were specified as: floor of 20 and normalization by mean, data with less than the minimal number of replicates of 4 were removed, and then followed by execution of proportion model and small sample model.
In addition, technical replicates showing 10% or greater absolute difference from the median value were removed, and only samples with at least two successful technical replicates were analyzed.
