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Four different partition schemes were applied to the sets of concatenated alignments; partitioned by gene, partitioned by codon position, portioned by gene with all third codon positions removed, an alignment consisting of only 3rd codon positions, and two different alignments of the translated amino acid sequences.
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You may remove an alignment dowel with a vice grips- if you chose not to have it resurfaced (bad idea).
Second, a segment of US-60/SH-11 US-60/SH-11 US-60/SH-11outeastofthe south on a straighter alignment; this new alignment also removed a concurrency with SH-18.
We further removed all alignments with more than five mismatches in the first 40 colors and a mapping quality below 20.
3067 bp of the alignment containing the control region between tRNA-Pro and tRNA-Phe were not well aligned due to high sequence divergence and were therefore removed, resulting in an alignment of 16,352 bp for phylogenetic analyses.
Cyanobacterial and diatom POR protein sequences used in the POR gene tree were aligned with MUSCLE [ 118] and incomplete sequences were removed, resulting in an alignment of 18 cyanobacterial sequences (18 genera) and 21 22 diatom sequences (15 16 genera) (Additional file 4).
Sequences from DRC collected in 1997 (1 ) were truncated to align with our C2V3C3 sequences, and gaps and ambiguous positions were removed, resulting in an alignment of 304 nt sequences; the first 60 nt represented the C2 region.
Duplicate and low-complexity reads were removed before alignment, resulting in a total of 10.2 million unique and high quality reads.
To remove the adaptors an alignment with the adaptors used during the sequencing process was done by Exonerate [ 102].
Sequences not aligned in the entire length were removed, and alignment was repeated.
This effectively removes a codon from the alignment, reflecting the uncertainty in reconstructing the translated amino acid associated with the deletion.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com