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When the weight is removed, air rushes back in, creating suction in the cleaning wand.
The next morning the cover slip was removed, air dried and sputter-coated with gold, mounted on the stub and examined in a LEO 435 VP scanning electron microscope.
Even in long treatments (e.g. nocturnal dialysis), the number of microbubbles was not decreasing towards the end of the treatment, indicating either other sources of microbubble generation or large 'stores' of initially not removed air somewhere in the blood tubing.
Each membrane was removed, air dried, and 1 ml of HCF (2 mg/ml of proteins) was spotted at one point of the filter and acted as control for a positive reaction before the membrane was washed in PBST (3 × 15 min) and blocked overnight at 4°C in 5% (w/v) skim milk in PBS.
Similar(56)
Gaseous N2 was applied to remove air from the flasks.
Before reaction, the autoclave was purged with nitrogen to remove air for five times.
The chamber was then sealed with upper and lower pedestals and evacuated to remove air.
Argon gas was purged through the reaction cell for 30 min before reaction to remove air.
The rolling action of the squeeze assists in removing air bubbles that could prove detrimental to the laminate performance.
The contents of the beaker were stirred to remove air bubbles, and solid particles were allowed to get wet.
Argon as an inert gas stream was used for vacating the reactor and removing air for several minutes.
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CEO of Professional Science Editing for Scientists @ prosciediting.com