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10% input sample (i) prior incubation was removed, adjusted to 45 μl with Laemmli sample buffer and boiled.
The supernatant was removed, adjusted to 300 mM NaCl, 10 mM imidazole, 67 mM NaH2PO4, pH 7.4 and loaded onto an Ni-NTA column (Qiagen) pre-equilibrated with 50 mM NaH2PO4, pH 7.4, 300 mM NaCl, 10 mM imidazole.
Exploration of this analysis identified that the cancer variable was non-significant only when spousal stress was in the model, but it became highly significant (p < 0.001) once spousal stress was removed (adjusted R =22, p < 0.001).
For each extraction step, a 60 μl sample of the supernatant was removed, adjusted to 1× NuPAGE loading buffer by the addition of 20 μl of 4× loading buffer and then boiled.
The adjusted HR for patients with 10 or fewer nodes removed was significantly reduced compared with that for patients with more than 10 nodes removed (adjusted HR, 0.579; 95% CI, 0.492 0.687; P < 0.001).
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Size effects were removed adjusting OLS separate regressions functions of PCTA, BrM, and DQ on BM.
This step allowed the establishment of grades which were significantly different from others and, therefore, to remove, adjust, or change pictures, which ensured the absence of discrimination.
With this step, we have been able to establish which grades are significantly different than others, and thus had the opportunity to remove, adjust, or change pictures, which ensured the absence of discrimination.
There she removed her, adjusted the basket at a rakish angle, and returned to the table.
Traffic and street lights are removed or adjusted to make space for maneuvering, and wind levels are recorded at each intersection.
Items that were unclear were either removed or adjusted.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com