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After the supernatant medium was removed, a solution containing 0.1 M. citric acid and 0.1% crystal violet was added to the cells and then the cells were counted using a hemocytometer (Burker-Turk, Tokyo, Japan).
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The effectiveness of removing a solution from the specimens' pulp space was tested in a preliminary test to this study.
As water is progressively removed from a solution of urea, the osmotic pressure opposing further removal progressively increases.
It can be removed with a solution that dissolves the polymer.
These can usually be removed with a solution of muriatic acid (available at home centers and tile supply stores) and water.
Next, the proteins and fats that make up the cellular and nuclear partitions are broken down and removed with a solution of detergent.
The Au and Au-Si alloy are removed by a solution of I2 KI: H2O (1 4 40).
The SR-30 XL soluble support material was removed with a solution of P400-SC Waterworks Soluble Concentrate (a caustic basic solution).
AAO of the AAO/Al membrane was removed by a solution of CuCl2 (0.1 M) and HCl (20 vol.%) for 10 min at room temperature.
In a first step, red blood cells were removed with a solution containing 12% (w/v) OptiPrep and 16.6 g/l methocel in 130 mM NaCl [26].
To ensure that at the end of the operation all the small pieces of nerve tissue around the artery are removed completely, a solution of 37% formaldehyde was applied for 1 minute on the surface of the artery.
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CEO of Professional Science Editing for Scientists @ prosciediting.com