The supernatant was removed, the volume reduced to 100 µL and measured with HPLC-ESI-MS.
By removing the volume information implicitly contained in SUVmean or SUV70 because of PVE, SUVrc and SUVdecon conveyed less information regarding the tumour response than when the volume information was implicitly included.
To evaluate the importance of the excluded volumes for discriminating actives from inactives for the new pharmacophore model we performed a test in which we removed the volumes in the same manner as in our previous study.
A numerical correction by a factor of 0.8 is employed to remove the apparent volume below particle due to projection.
A numerical correction by a factor of 0.8 is employed to remove the apparent volume below the particle due to projection.
However, there exists a minimum cutting length, (xc min below which droplet generation ceases [Additional file 4], which makes it impossible to completely remove the additional volume to a dispensed droplet with the conventional approach.
No slices with any hippocampus were used to remove the possibility of volume conduction of hippocampal network activity.
Similarly, the 5 mm slices with 5 mm gaps remove the possibility of volume averaging or slice crosstalk [ 3] while fairly representing interleaved acquisition mode which is a clinically feasible option in MRI.
After a total of 15% of the EBV had been removed, the intravascular volume was then replaced, also in three 5% of the EBV aliquots, using an equal volume of 6% hetastarch in lactated electrolyte solution (Hextend, Hospira, Inc., Lake Forest, IL).
To ensure that our results were not due to undue influences of results concerning the large volume of the neocortex, we repeated our analyses after removing the neocortex volumes from the "remaining brain volume" variable used to correct for allometric effects (Additional files 5 and 6).
