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Appropriate barrier filters at 500 and 800 nm remove the reflected light with unchanged wavelength while allowing only the light emitted by the dye upon stimulation to pass through.1 A detailed protocol for anesthesia and conventional imaging is described elsewhere [1].
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Every element in the boron group has three electrons in its outermost shell (so-called valence electrons), and for each element there is a sharp jump in the amount of energy required to remove the fourth electron, reflecting the fact that this electron must be removed from an inner shell.
All three of these protocols perform optical sectioning, meaning that they remove light reflected directly to the camera by points off the focal plane.
The remaining reflected light appears as the complementary colour of the removed wavelength (e.g., if red light is removed by destructive interference, the reflected light will appear as cyan).
Even though the long-pass filter removes the majority of the reflected STED laser light, it was attenuated further by several neutral density filters adding up to 3.3 4.6 OD to avoid detector saturation.
The impending constitutional referendum would remove the nationality clause, which would reflect Ivory Coast's multiethnic population and promote the inclusion of all Ivorians, regardless of their origins, in political affairs.
We confirmed with manual inspection on two sample projects that this simple heuristic was able to accurately remove the undesired files, since it reflects (in our sample) the common developer practices of naming test files with a "Test" prefix of suffix, or placing test code in a separate directory named "test".
The upper lamina is irregular structured and thus removes the intrinsic directionality of the reflected light and makes the color mostly angle-independent.
So, the current situation removes the motivation to reflect on the past and improve the future.
In particular, it removes light reflected to the camera, whether by points on or off the focal plane, after multiple bounces.
We chose to remove that option (reflected in the Supplemental Material: DCI), which raised item 13 discrimination from 0.07 to 0.11.
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