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We chose the lower limit of 2° to remove the Pg phases and the upper limit of 12° to include only the observations along the linear portion of the travel time curve, as well as to avoid arrivals from the deeper part of the upper mantle.
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The attenuation of the Pg phase propagating in the crust is very strong because large Pg-to-S conversion occurs at the free surface and the Moho (shown in green between Pg and Sn in the T = 80- and 200-s frames), which removes the Pg wave energy from the crust down to the mantle.
The studio eventually agreed to remove the language and an edited version of the film received a PG-13 rating.
The electrodeposited PG/NF electrodes were then immersed in deionized water to remove the unwanted GO and LiClO4.
Finally, the purified PG is obtained by treating the samples in a boiling water bath for 10 min and centrifuging it at 13,000 ×g to remove the contaminating lysozyme.
After 4-hour trypsin treatment, the trypsin solution was removed and the PG-degraded specimens were washed with PBS solution for three times.
Cellular PG were assessed by removing the cells with Triton X-100/NH4OH X-100/NH4OH
Disks were incubated for 12 h in phosphate buffered saline (PBS, IS = 0.15 M, pH = 7.4) at 37 °C in the presence of 0.1 mg/mL bovine pancreatic trypsin (Sigma-Aldrich, St . Louis MO) to remove proteoglycans (PGs) without interrupting the macroscopic and microscopic structure or static tensile properties of the collagen network.
For immunohistochemical analysis of MMP-induced neoepitopes, sections were deparaffinised, rehydrated and digested with chondroitinase ABC (Sigma; 0.25 U/ml in 0.1 M Tris-HCl, pH 8.0) for 1 hour at 37°C, to remove chondroitin sulphate from the PGs.
Therefore, removing H3PO4 from PG or transforming it into a phosphate is an effective approach to render PG suitable to serve as the added gypsum of CSA cement.
(Scott) * 'THE HITCHHIKER'S GUIDE TO THE GALAXY' (PG, 103 minutes).
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