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Nuclear transfer is a cloning method in which researchers take a normal egg, remove the nucleus, which contains most of the genetic information, and replace it with the nucleus from the cell being cloned.
The scientists take an egg with damaged mitochondria, remove the nucleus (and the DNA it contains) and transplant it into a second, donor egg, whose nucleus has been removed but whose mitochondria are working normally.
The Massachusetts company simply wants to remove the nucleus from a female donor's egg, insert a cell from the skin of another donor, and then stimulate the egg to reprogram the genes of the skin cell to start growing into a blastocyst.
For instance, cloning an animal requires that researchers first remove the nucleus of an egg cell.
When scientists attempt to remove the nucleus from the primate egg, they also end up removing or destroying the spindle proteins, which makes it impossible for the cell to divide and grow into a human.
The homogenate was transferred to centrifuge tubes and centrifuged at 1,000 g for 10 min to remove the nucleus.
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Bossilier testified that scientists working with the project are currently practicing removing nuclei from cattle eggs, but have not yet attempted to remove the nuclei from human eggs.
Briefly, homogenates were centrifuged at 15 800 × g for 10 min to remove the nuclei.
The cell lysates were centrifuged at 15,000 ×g for 5 min at 4°C, to pellet and remove the nuclei.
The pellet was then resuspended in 200 μl fractionation buffer, homogenized with a ball-bearing homogenizer and centrifuged at 900 × g for 5 min to remove the nuclei.
One hundred brains from 1-week-old mice were homogenized in 200 ml of buffer (TBS containing a protease inhibitor cocktail) and then centrifuged at 500 × g for 10 min to remove the nuclei of the cells.
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