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Samples were centrifuged at 4000 rpm for 10 min to remove the medium.
The cultivated cells were harvested by centrifugation and washed with distilled water to remove the medium components before being lyophilized.
Briefly, after the end of incubation period, the plate containing the treated and untreated cells were inverted or aspirated to remove the medium.
Rooted plantlets were removed from the culture vessels, rinsed with water to remove the medium, and then transferred to plastic pots (9 cm diameter) containing a mixture of peat soil: perlite: vermiculite (1 1 1 v/v) in the greenhouse.
After 90 minutes, both sets of cultures were transferred to centrifuge tubes to spin down cells and to remove the medium and /or the virus.
After incubation, the plates were inverted on a paper towel to remove the medium.
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The more removed the medium of communication, the easier it is for us to set aside our compassion.
After cells were attached, removed the medium, and fed cells with 5 mL of fresh medium.
After removing the medium, Bright-Glo luminescence substrate (Promega) was added, and luminescence was measured.
After removing the medium, 10 mL of 12 mM MTT solution was added and incubated for a further 4 h.
After removing the medium, the cells were washed three times with PBS in order to detach the free csMSN.
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