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At the end of hybridization time carefully remove the array from water bath.
RMA was used to remove the array signal background, followed by quantile normalisation to correct for inter-arrays global differences and by gene level summarisation.
Robust Multichip Analysis (RMA) methodology [ 48] was used to remove the array signal background, followed by quantile normalisation to correct for inter-arrays global differences and by miRNA level summarisation.
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It removes the array of options, like advanced markup features that you'll see on WordPress and other established platforms, to focus writers on core ideas.
However, we could not successfully remove the nanocolumnar array from the glass substrate onto which it was directly deposited by doing this, the two types of imperfections mentioned above being present in the first self-standing flexible mirror we prepared.
Next, the ComBat algorithm was used to estimate and remove the technical bias (array type, scanner and vehicle) from the normalized data matrix [35].
The deconvolution approach to mapping acoustic sources (DAMAS) is employed to remove the effects of the array point spread function.
For those datasets whose CEL files are available at GEO, the data were first subjected to quality assessment by AffyQualityReport to remove the poor quality arrays and then to RMA[ 36] processing for data normalization.
We removed the outlier array S54 that leads to a dataset with 97 samples and 1213 genes.
Background-corrected intensity data were normalized using the Print-tip loess method to remove the bias within each array and the A-values quantile normalization (Aquantile) to remove the bias between arrays.
Expression data was filtered to identify genes expressed on the BeadChip at >0.99 confidence (16,585 genes), and normalized per chip using non-linear Normalize.quantiles or VSN normalization to remove array to array variability (Bioconductor Project [ 43]).
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