Sentence examples for remove selection marker from inspiring English sources

In the second step, the counterselection was performed to remove selection marker using a reporter gene cassette without a cI- spc cassette to generate two transgenes, Tg-110 and Tg-220.

Moreover, with the help of the Cre-LoxP system we removed the selection marker gene which is absolutely nonessential for expression of target proteins.

You may remove selections by dragging them off your projects.

A random sampling method removes selection bias.

Transient expression of Cre recombinase was then used to remove the drug selection marker, leaving a single LoxP site between exons 21 and 22 and not affecting the LoxP site between exons 12 and 13.

Cre- loxP recombination system has been an integral part of mouse genome manipulation, initially to remove the selectable marker and thus eliminating 'the selection cassette effects' and subsequently to overcome certain deficiencies associated with ubiquitous gene deletions.

This prompted us to generate FLPo transgenic mice, which turn out to be very efficient in removing the FRT-flanked selection marker.

In addition, to improve the final engineered product, we utilised Flp recombinase to remove the unc-119 selection marker, in somatic cells, producing clean knockouts in these cells.

The PGK neo selection marker was selectively removed from the NR1 3lox allele by transient Cre (cause of recombination enzyme -recombinase enzyme -recombinasectroporation of thexpressionant ES cells with moderate amoupon (10 µg) of Crelectroporationncofing plasmid pMC-Cre-recombinase (Gu ethel., 1993).

Coupled with their natural insect specificity, safety concerns can also be mitigated by using safe effector proteins with selection marker genes removed after transformation.

The selection marker was removed from the A1B1 and A1B2 cell lines by transient transfection of Cre recombinase and selection of the cells on gancyclovir (Figure 5A).