Exact(7)
The alignments of these proteins were concatenated into a single alignment, which was then trimmed to remove positions with gaps in more than 50% of the organisms.
Aligned sequences were filtered to remove positions with gaps as well as positions directly adjacent to gaps.
For both analyses, we filtered data to remove positions where less than 75%% of individuals were genotyped for each of the two taxa being compared.
The paired alignment is then filtered to reduce redundancy to 90% sequence identity and to remove positions that have more than 75% gaps.
Phylogenetic trees were constructed by neighbor-joining analysis of nucleotide alignments using Jukes Cantor distance correction and a column filter to remove positions of uncertain homology.
Further, additional filtering used to remove positions with low number of alternate alleles (Section 2.4) is likely to remove such false SNPs.
Similar(51)
The central 64 pots in the dense stand were used for measurements, and these pots were rotated every 10 d to remove position effects.
Following [23], for each genotyped locus, we defined haplotypes according to sequence identity after removing positions for which only one sequence varied from the consensus.
Because Fastloc GPS location estimates vary, with a higher number of satellites generally yielding more accurate locations, we removed positions with <5 satellites [reported field-test spatial errors (mean ± S.D .: 8 satellites, 26 m ±19.2; 5 satellites, 64 m ±79.4] [13].
Nucleotide positions on the x-axis mark the removed positions upon transition to a reduced model.
After removing positions with no residue variation in all sequences, and positions that only contained gaps in all microsporidia species, the alignment contained 25,640 positions.
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