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Further, we have successfully developed a new plasma native peptidomic technology that allows to efficiently remove plasma high abundant proteins to comprehensively identify circulating native peptides using mass spectrometry9,10,36,37.
Briefly, 6 8.5 ml peripheral blood, collected into a tube containing acid citrate dextrose anti-coagulant (Becton Dickinson, Franklin Lakes, NJ, USA), were subjected to centrifuging to remove plasma, followed by centrifuging again on the top of non-hematopoietic cell separation matrix to remove RBCs.
This was repeated three times to remove plasma and buffy coat.
For erythrocytes, the blood samples were centrifuged at 1200 g during 10 min to remove plasma and buffy-coat.
Platelets were pelleted from PRP by centrifugation at 1100×g for 10 min and washed three times as described above to remove plasma proteins.
Because PPTs require less specimen processing (e.g. no need to remove plasma from cellular components of sample), their use may improve lab technician safety and efficiency.
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Hemofiltration removes plasma water and soluble components below 25 kilodaltons.
The CTC-iChip first separates nucleated cells from other blood components (i.e., "debulking" by removing plasma, platelets, RBCs, and free beads) by deterministic lateral displacement, before aligning nucleated cells in a single file by inertial focusing and magnetically deflecting bead-labeled leukocytes under continuous flow.
Those beads significantly removed plasma cytokines and chemokines and decreased BALF-to-blood chemokine ratios, resulting in decreased leukocytes recruitment into the lung.
Removed plasma was substituted with fresh frozen plasma (FFP) in a 1 1 ratio in 22 patients.
Free quinine was obtained by removing plasma proteins by ultrafiltration using Millipore Centrifree Centrifugal Filter Units.
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