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(iv) Remove mismatches: "No".
Further studies, in particular to elucidate if there is any MMR activity in the protein complement of H. salinarum NRC-1, are necessary to differentiate if the low mutation rate observed in this organism is the result of intrinsic properties of its DNA polymerase or if DNA repair activities are recruited to the replication site to remove mismatches.
The exonuclease domain of WRN has proofreading activity that can remove 3' mismatches following misincorporation of nucleotides by pol β, and thus potentially enhance the fidelity of BER [38] [40].
Normally, MMR (Modrich, 2006; Hsieh and Yamane, 2008; Jiricny, 2013) is a high fidelity process that operates post-replicatively on the nascent DNA strand to remove mismatches that have escaped the proof-reading activity of high fidelity replicative DNA polymerases.
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TDG was first shown to remove mismatched pyrimidines from G U and G T pairs in dsDNA.
The function of MMR is to ensure the fidelity of DNA replication by removing mismatches produced during DNA synthesis [11], [12].
To test our hypothesis that the gene block between duplicated rrnS failed to amplify in Yu et al.'s study, we synthesized the three primer pairs used by Yu et al. (after removing mismatches based on our C. hongkongensis sequences to improve specificity).
Escherichia coli has a correction system which removes mismatched bases from DNA1.
This is supported by the previous observation that removing mismatch repair decreases the position effects on mutation rate (Hawk et al. 2005).
For the CMOD control loading procedure, a loading rate of 2 kN/min was used to remove geometrical mismatches and gaps until the load reached 2 kN.
For the loading procedure of the stroke control, a 5 kN load was gradually applied onto the notched beam using a rate of 2.5 kN/min in order to remove geometrical mismatches and gaps.
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