Sentence examples for remove membrane from inspiring English sources

The oncologically relevant forms of ALK are most commonly fusion proteins [76, 101] that remove membrane attachment and render ALK constitutively active.

Endocytosis is required in the AS to remove membrane during apical constriction, but is not essential in the epidermis.

Cell suspensions were then filtered (100 µm) to remove membrane aggregates, rinsed twice with PBS 5% FCS 0.5 mM EDTA.

We also observed apical membrane folds that progressively fuse to form thick projections, which could be another mechanism to rapidly remove membrane from the apical surface area (Figure S3F I).

Our results suggest that the activity of Rab5 is required to remove membrane from the AS cells during apical constriction, whilst Rab11 exhibits an opposite effect, being required to increase the apical surface of cells.

Cells were lysed in 1.5 ml lysis buffer (50 mM Tris pH 7.5, 5 mM EDTA, 300 mM NaCl, 150 mM KCl, 1 mM DTT, 1% Nonidet P40, and Roche Complete™ protease inhibitor added at the time of use) at 4°C. 200 µl of whole cell lysate was treated with 40 µl Concanavalin A beads (Amersham) as previously described [47], and rotated for 4 hours at 4°C to remove membrane associated β-catenin.

Peel and remove membranes from sections.

The cells were rinsed twice with 1 mL ice-cold Glycine-buffer and incubated for 5 min at RT to remove membrane-bound radiolabelled peptide (membrane-bound fraction).

Washing, along with a process to remove membranes prior to alkaline solubilization, was very effective in preparing a substrate for protein hydrolysate production, in which fishy odor and taste could be significantly lowered.

Mixtures were subjected to three steps of centrifugation (10 min, 10,500×g, 4°C) to remove membrane-bound tracer and rinsed with 0.5 mL ice-cold Tris-HCl buffer.

For detection of sAPPα and β (IBL antibody #27724 & #27722, respectively) an additional 45 min spin at 100,000 g was used to remove membranes prior to SDS-PAGE.