Sentence examples for remove medium from inspiring English sources
Suspensions (1 mL) were washed by deionized water to remove medium on the mineral surface.
Isolated cells (106 cells per assay) were centrifuged at 300× g for 5 min, washed in sterile PBS without Ca2+ or Mg2+, and again centrifuged to remove medium traces.
Following centrifugation to remove medium, cells were resuspended in 0.2× phosphate buffered saline, boiled for 10 min, and mixed with 0.1 volume of 10-mg/ml proteinase K (Sigma, Marlborough, MA), and the mixture was incubated for 30 min at 55°C.
Remove medium and wash with PBS twice.
This was repeated three additional times to remove medium contaminants.
After removing medium, 0.318 mM 4-MUNANA (2′- 4-methylumbelliferyl -α-D-N-acetylneuraminic acid; Biosynth Intl).
After removing medium, cells were fixed in 4% buffered paraformaldehyde (pH 7.4) for 30 min. Slides were rinsed twice in PBS, pH 7.4.
Removed medium was replaced with sterile COMBO medium.
Removed medium was frozen and stored at −80 °C.
Complexes were removed, medium replaced and cells left for 24 h.
