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And cells were washed to remove media.
You can remove media from the NAS adapter without disconnecting power.
The fully expanded mycelial mat was harvested aseptically and washed with sterile distilled water to remove media components.
In contrast, manual operators could remove media more completely (yielding a larger XTT signal) by tipping the plate and pipetting from well corners.
Cells were washed and harvested by trypsinisation, centrifuged to remove media, and washed twice in cold PBS.
Briefly, approximately 2×106 C2 ES cells were thawed onto a 6-mm tissue culture-treated dish containing irradiated MEFs, expanded over three passages, trypsinized using 0.05% Trypsin (Invitrogen) and centrifuged to remove media.
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After added 10 µl media binding reagent and 1.25 µl Annexin V-FITC, cells were incubated at room temperature for 15 min in the dark, then centrifuged and removed media.
After 90 min, we removed media that contained sporozoites that had not infected the cells and added fresh media.
At the end of insulin secretion experiments, inserts with islets were removed, media were collected, and frozen at −20 °C for insulin assay.
Removing media files using the method above only removes it from Winamp's library and does not delete the actual file.
After 72 h of treatment, mifepristone-containing media was removed, and media for all cells was replaced with control media.
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