Moreover, the lack of DC specific markers has meant previous flow cytometric analysis relied on negative selection using a large panel of antibodies to remove lineage-specific cell populations.
CD34+ cells were enriched by removing lineage committed cells using a cocktail of antibodies with the RosetteSep system according to the manufacturer's instructions (Stem Cell Technologies, Vancouver, BC).
Further work has instead grouped these KIRs into lineage II and removed lineage IV [ 47].
Despite this large variation, the stringent selection procedures used to extract orthologs make for reliable comparisons as evident by the facts that 1) the ranges of K a values are equivalent across lineages and 2) by removing the lineage with the smallest ortholog set the trend did not change.
To achieve this, we removed the lineages that led to the root-node of the maximum clade credibility (MCC) tree estimated in BEAST from the complete data set (Additional file 4: Figure S1).
Strategies for mitigating LBA artifacts include removing LBA lineages (Duvall and Bricker Ervin 2004; Hampl et al. 2009) or deleting fast-evolving sites or genes (Hajibabaei et al. 2006; Goremykin et al. 2009; Hampl et al. 2009; Inagaki et al. 2009).
Recent studies have revealed that TLR signaling elements and AID function not only in peripheral B cells to help mediate effective antibody responses to foreign antigens, but also in the BM to help remove autoreactive B-lineage cells at a very early point in B-cell development.
In this counterfactual situation Joe is still a member of his species so long as we do not remove him from the lineage and gene pool of that ring species.
To remove the effect of lineage number, we compared groups of trees with the same number of diversification shifts.
At the end of day 12, the mixed cultures were shaken overnight (14 16 h) at 250 rev./min in order to remove supernatant containing OLG lineage cells and microglia.
The multi time RJMCMC methods are akin to back filtering methods of particle filters in that they can occur over multiple time points and at any current or previous time and are generally defined by moves that affect the tree of the lineage, such as: adding a branch to the lineage, or removing a branch from the lineage.
