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The alignments were pruned to remove gap sites present in 50% or more of the aligned sequences.
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We applied the pairwise deletion to remove gaps as our sequence lengths are varied, and a complete removal of the gaps is not a good choice as it eliminates a large portion of phylogenetically meaningful sites from consideration.
(ii) Remove gaps and inframe stop codons: "Yes".
Multiple sequence alignments were generated using ClustalW [57] and edited using Bioedit [58] to remove gaps.
Full length sequences for all taxa were aligned with Muscle 3.6 [48] and edited manually in the case of clear errors and to remove gaps.
To construct phylogeny, sequences were first aligned with ClustalW, edited in BioEdit to remove gaps, and then Neighbor-joining trees were constructed and drawn with TREECON for windows.
The resulting MSAs were manually edited to remove gaps and ambiguously aligned regions.
The alignment was trimmed to remove gaps in most of the sequences.
PAL2NAL was also used to remove gaps and in frame stop codons.
Codon alignments were subsequently imported into BIOEDIT and manually edited to remove gaps and regions of poor homology.
The sequences were assembled using BioEdit v7.2.0, after which they were manually edited to remove gaps or ambiguously aligned sites.
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