Sentence examples for remove cytoplasmic from inspiring English sources

HeLa cells were incubated with ethanol or 2 mM DEM for 4 hours, extracted with digitonin to remove cytoplasmic proteins and boiled in 40 mM Tris HCl pH 8.0, 1% SDS, 50 mM DTT containing 1 mM NaN3, 2.5 mM NaF and a cocktail of protease inhibitors (aprotinin, pepstatin, leupeptin, each at 1 µg/ml).

This inability was caused by an impaired capacity of the mitochondria to remove cytoplasmic calcium.

To remove cytoplasmic membrane, 0.05 ml of 1% (v/ v) NP-40 solution was added, and the sample was vortexed for 3 times (2 sec per vortex).

The pellet obtained after the first centrifuge, containing nuclear material, was washed 3 times with lysis buffer and centrifuged, in order to remove cytoplasmic protein traces.

Interphase cell preparations were incubated at 37°C for 10 min and then treated with 300  μl ml−1 HCl pepsin, pH 2.7 3 (Sigma, Dorset, UK) at 37°C, to remove cytoplasmic proteins, hence improving probe penetration.

Nuclear extraction was performed by adding 500 μl PIPES buffer (5 mM Pipes pH 8.0, 85 mM KCl, 0.5% Nonidet P-40, protease inhibitors), incubating for 10 min on ice and removing cytoplasmic components by centrifugation.

The cellular lysate was centrifuged at 20 000 g for 10 min and 400  μl of the supernatant were removed (cytoplasmic fraction) and diluted 1 : 10 with incubation buffer, absorbance was measured at 405 nm, the substrate solution was used as blank.

Tissues that putatively have the highest sulfation activities are those that are affected most severely by loss of the ubiquitously expressed 3′,5′-bisphosphate nucleotidase (BPNT1) phosphatase, the enzyme that removes cytoplasmic PAP, the otherwise toxic by-product of sulfation, by degrading it into AMP and phosphate.

To further demonstrate this, we permeabilized the cells post-treatment to remove soluble cytoplasmic and nuclear proteins [49] and conducted immunostaining as above.

For PBS-TX extraction, Neuro2A cells were first treated with Digi Buffer (1 × PBS, 1 m m EDTA, 1 m m DTT, 0.01% digitonin) for 5 min at 4 °C to remove the cytoplasmic fractions.

After prepermeabilization of the cells with saponin and extensive washes to remove the cytoplasmic pool of proteins before fixation, a pool of SLY1-GFP was found to colocalize with the ER exit sites specific COPII component SEC31.