Aliquots of 1.5 ml were then removed, chilled, mixed with 1.5 ml of butanol and centrifuged at 4,000× g for 10 min. The organic fraction was recovered and the OD535 was measured spectrophotometrically.
Each tube was removed, chilled on ice (10 min), harvested by brief centrifugation, cells washed (2 × 1 ml ice-cold ddH2O), resuspended (50 μl, ddH2O) and electroporated with an appropriate, purified (Promega, Wizard) RT-cassette-containing PCR product (500 ng).
Remove and chill in the fridge.
Remove and chill. 2 Cover the pork with water in a casserole.
To allay these fears and remove the chill from investment, President Obama and congressional leaders should forcefully renounce the act now.
Her manner is sensuous but slightly removed (not chill, removed) in a 21st-century space where the African visions and hippie dreams of the 60's meet.
Consecutive victories have removed the chill that permeated the team's offices and meeting rooms when the Giants' record was 0-6.
But in this misconceived revue, directed by Eric D. Schaeffer, her magnetic warmth is being put to the cause of removing the chill from the songs of Stephen Sondheim.
Decommissioned and abandoned plants lie like ghosts on the landscape ("Chernobyl broke our backs," a disgruntled worker says), though the rebirth of one of these as an amusement park barely removes its chill.
We awakened to a bright dawn, a wind-ruffled lake and below-freezing temperatures, but the fireplace and the sun streaming in through picture windows and glass doors soon removed the chill.
