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After "wounding", cells were washed with PBS to remove cell debris and incubated with full medium.
Presence of high salt in lysing solution helps to remove cell membranes, bulk of proteins, cytoplasm and nucleoplasm.
The lysate was centrifuged at 20,000 ×g for 30 min at 4°C to remove cell debris.
After that, the whole plate was centrifuged at 250 × g (1,300 rpm) for 10 min to remove cell debris.
To remove cell debris, samples were centrifuged at 2000 rpm for 10 min, followed by resuspending in double distilled water.
The suspension was centrifuged to remove cell debris.
The homogenates were briefly centrifuged to remove cell debris.
The supernatant was collected and filtered over 0.2 µM filters to remove cell debris.
The cell extract was centrifuged for 30 min at 8,000× g to remove cell debris.
When disaggregation was complete, cells were centrifugated (5 min, 300g) to remove cell debris.
After sonication homogenized tissue was centrifuged at 1500 xg for 10 min to remove cell debris.
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