Sentence examples for remove cassette from inspiring English sources
Always remove cassette carefully, making sure never to force it.
Remove cassette from player when not in use and return to plastic case before storing or stacking.
Cleverly, Dickinson et al. (2015) included the Cre gene under a heat-shock inducible promoter in their "self-excising cassette," which allowed researchers to use hygromycin selection for the knockin, followed by heat shock to remove the cassette.
To make pT2K-GFAP-EGFP, pT2K-BI-EGFP was digested with EcoRI to remove the cassette containing the TRE sequence.
By flanking the markers with LoxP sites, Cre-mediated excision can be used to remove the cassette.
pT2.CMV-hKO (5.1 kb, see Additional File 1): pT2AL200R150G [ 13] was digested with XhoI/ BamHI to remove the cassette containing the EF1α promoter.
pT2MIK (6.7 kb, Figure 1C): pT2AL200R150G [ 13] was digested with XhoI/ BglII to remove the cassette containing the EF1α promoter-eGFP-polyA sequence.
Mars stops the tape player, removes the cassette tape and begins singing his song.
Subsequent transfection with the FLPe expression vector removed this cassette to generate Esrrbfl/+ ESCs.
We also constructed a second single shRNA expression vector pENTR-hH1 by removing hU6 cassette from pENTR.hU6.hH1 with NotI/ EcoRI.
LoxP/Cre-mediated cassette excision was used to remove the PGK/Zeo cassette from the Zeocin-resistant targeted intermediate and the AAV integrated non-targeted cells.
