Sentence examples for remove bound from inspiring English sources

It is conventional to use high salt buffers (for example, 300 1000 mmol/L NH4Cl) to remove bound factors or other undesired proteins to obtain active ribosomes or ribosomal subunits through centrifugation (Blaha et al., 2000; Rodnina and Wintermeyer, 1995; Selmer et al., 2006) or chromatography (Jelenc, 1980; Maguire et al., 2008) based purification techniques.

The proteins were dialyzed extensively to remove bound putrescine.

The pellet was then washed twice with 0.9% NaCl and resuspended in 0.05 M NaCl to remove bound proteins.

10 mM glycine pH 2.5 was used at the end of each cycle to remove bound TCR and ligand.

In immunosensing, by contrast, it is practically impossible to remove bound antigens from antibodies without damaging the antibodies, because both antibodies and antigens are proteins in nature.

Phagocyte monolayer was washed with PBS to remove bound, nonengulfed apoptotic cells and harvested by scraping at 0, 0.5 and 5 h time points after apoptotic cell addition.

For fluorescent transferrin binding and uptake assays, cells were preincubated in DMEM, 25 mM Hepes, 0.5% BSA at 37°C for 1 hr to remove bound transferrin.

hMDM monolayers were washed vigorously with ice-cold PBS to remove bound but non-ingested neutrophils then lysed with 0.1% hexadecyltrimethylammonium bromide (15 min, 37 °C).