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Reads that started with the PstI overhang sequence (TGCAG) after barcode removal were accepted, quality trimmed to remove bases of quality Phred < 20 from the 3'-end (distal to the PstI site), and then shortened from the 3'-end to a standard length of 64 bases.
He also said an independent Scotland would demand that Britain remove bases on the River Clyde for Britain's fleet of nuclear missile submarines, and pay for environmental cleanup costs that experts have said could run into the billions.
An additional filter was applied to remove bases with low mapping quality scores.
The FASTX toolkit (fastq_quality_trimmer) was applied to remove bases with Phred quality scores below Q = 13 from the end of each read.
An alternative hypothesis proposes that estradiol can be metabolized to quinone derivatives, which can react with DNA and then remove bases from DNA through a process called depurination.
7. If the Tm of the forward primer is above 59°C remove bases from the 5' end one at a time and calculate the Tm.
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Remove base structure.
Dissolve PVA with hot water and remove base structure.
To set clear range of the sequence quality value method (Remove base from the ends until fewer than 4 bases out of 20 have QVs<20) was used.
Finally, to accommodate motif insertions RNA-MoIP can remove base pairs.
For the other models, we removed bases with base or mapping qualities <10.
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