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To remove assembly artefacts and transcriptional noise, we filtered transcripts with a Fragments Per Kilobase Million (FPKM) <1, retaining 26,852 transcripts.
The genome sequence of Actinoplanes sp. SE50/110 was known before, but was resequenced in this study to remove assembly artifacts and incorrect base callings.
And then CD-hit [ 54] and CAP3 [ 55] were used to remove assembly redundancy by setting global sequence identity in CD-hit to 1, the minimal overlap length and percent identity in CAP3 to 100 bp and 99%.
To remove assembly artefacts, such as redundancy, only the contigs showing 85- to 117% variation between number mapped and number assembled reads percentage were retained as high quality contigs in CamemBank01.
Remove assembly.
MILWAUKEE, March 2 (Reuters) - Wisconsin Republican lawmakers will attempt to remove Assembly Majority Leader Bill Kramer from his leadership post after two women accused him of sexual harassment last week, according to local media on Sunday.
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To remove low quality sequences, we filtered all PUT sequences to remove assemblies that were shorter than 250 nt, that had >10 N's, or that had >50% low-complexity sequence as determined by the seg low-complexity filter program.
Table 1 Assembly statistics information Raw assembly Duplicate removed assembly Trinity 'genes' 27,389 24,000 Trinity transcripts 31,068 25,234 GC content 40.84 40.71 Median contig length (bp) 722 622 Average contig length (bp) 1269.6 1160.9 Total assembled bases (bp) 39,444,068 29,294,166.
However, when the light was removed, assemblies with different OD600 values showed varying degrees of disassembly (Fig. 4c).
In both cases, we were able to remove some assembly errors, while not decreasing the error-corrected N50 values.
Remove tub assembly from washer.
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