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The whey fraction was filtered through a 0.4 μm PVDF filter and then a 0.2 μm PVDF filter to remove any further complex particles, and also to allow free flow of the sample through in the subsequent filtration step.
To remove any further residual confounding by season, we included indicator variables to represent months with average monthly temperatures above 10°C (50°F) during the baseline recruitment period (all year for Los Angeles; May to October for Chicago and St. Paul; and April to October for the remaining three sites).
Scrub the fireplace brick with the dilute cleaner to remove any further stains.
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In so doing, Facebook instantly removed any further conversation about RSS.
We also removed any further potential biases in gene expression using a Euclidean based similarity normalisation approach (see methods) and found that the significant enrichment in essential genes remained after this bias correction.
After the desired pH was obtained, the alkaline extract was filtered to remove any precipitate and further washed with equal volume of hexane thrice.
We assessed correlated paternity within pods (rppm) at a site within the main range (MA2; Fig. 1) so as to remove any bias from further assessment of paternity and mating system parameters.
Nixon cultivated that idea because "charges of 'sore loser' would follow me through history" and remove any possibility of a further political career - but Nixon and his allies did mount a massive challenge.
Cellular material was pelleted by centrifuging the supernatants for 1 min at 16,100×g and then 1 mL of each clarified supernatant was passed through 0.45 µm filters to further remove any cellular components.
RNA extraction was performed according the Trizol manufacturers' protocol, followed by a sodium acetate precipitation to further remove any contaminants.
To further remove any minor contamination, an ion exchange Mono Q column was employed (Supplementary information, Figure S1).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com