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To assess Cu II) removal, samples were collected at different time intervals.
Following bud and flower removal, samples were immediately frozen in liquid nitrogen to avoid RNA degradation.
After removal, samples were macrodissected by pathologists and frozen within 30 min of removal.
After surgical removal, samples were immediately snap-frozen in a liquid nitrogen container.
Our previous study showed that CNB had a high concordance rate in evaluating molecular subtype status compared with those in surgically removal samples (SRS) [ 4].
During July, from 1996 to 2008, the population abundance was estimated by a standardised temporary removal experiment [ 32], with three removal samples obtained every other day (i.e. the total sampling period was 96 hours).
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Open image in new window Fig. 5 Core removal, sampling, testing, and penetration.
Removal sampling methods are commonly used, but imperfect detectability is rarely accounted for in the analytical framework.
Using a removal sampling design, we studied the performance of a Bayesian approach to estimate both sampling rates and abundance under the assumption of a closed population.
A second blood sample was collected 28 days after tick removal (sample 2).
Following biopsy removal sampling sites were sealed with Michel surgical suture clips (Dunlops, UK).
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