Sentence examples for removal of sequence from inspiring English sources

It supports adapter detection and removal, demultiplexing of barcodes, paired-end data, a range of read architectures and the efficient removal of sequence redundancy.

Therefore, the removal of sequence contaminants is a necessary and required step for all sequencing projects.

Based on the evaluation results, we adopted BWA-SW for the removal of human sequence contamination from metagenomes and developed DeconSeq, a robust framework for the rapid, automated identification and removal of sequence contamination from longer-read datasets.

Further processing of the raw deep sequencing data consisted of: i) removal of sequence tags with a non-matching 5' or 3'-adapter or resulting from adapter self-ligation, ii) adapter trimming from the remaining tags, and iii) selection of sRNA sequences ranging in size between 16 and 26 nt.

Removal of sequence tags with a non-matching 5'- or 3'-adapter or resulting from adapter self-ligation yielded a set of 3,922,430 (75.4%) high quality sequence tags, each containing a small RNA insert and perfectly-matched adapters in the expected configuration.

These assembly statistics alone do not entirely reflect the impact of normalization on genome assembly, however: de novo assembly of the non-normalized library was generally impossible without the prior removal of sequence reads from the most highly overrepresented regions, whereas assembly of the normalized library was possible without this additional step.

Notably the similarity between samples (Table S2) was not greatly influenced by the removal of sequences.

In the absence of Rev, multiple splicing events lead to the removal of sequences encoding Gag and Env.

To predict the effect of nonsynonymous (missense) mutations on protein function, the SIFT software was [57] used with default parameters (median conservation of sequences = 3; removal of sequences >90% identical to the query).

After removal of sequences with deletions or premature stop codons, a total of 258 sequences (126 corresponding to the 5' fragment and 132 corresponding to the 3' fragment of SU) corresponding to 139 complete env gene variant sequences, remained.

Illumina reads were 76nt (GAIIx) or 101nt (HiSEQ 2000) after removal of sequencing adapters.