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This is likely due to the removal of cells, cellular debris and bacterial proteins during centrifugation process.
The decellularization procedure described here lead to the preservation of the native extracellular matrix architecture and the removal of cells.
Scanning electron microscopic examination of fibroin nets after removal of cells did not demonstrate any changes to the fibroin structure.
Regardless of product source, removal of cells, cellular debris, aggregates and precipitated material is usually necessary prior to purification.
Critical to the HADM ability to remodel and integrate into the host tissue is the removal of cells while maintaining an intact extracellular architecture.
Examinations by TEM showed that the characteristics of collagen fibers in the ADM were retained after complete removal of cells present under optimal conditions defined in this study.
Using on-the-fly addition and removal of cells during the simulation, it allows the description of the atomic displacements induced by a cascade.
Cell-based therapies require removal of cells from their crowded physiological context and propagating them in dilute culture medium to attain therapeutically relevant numbers whilst preserving their phenotype.
The inhibition of complement may be very important in a variety of these diseases because as the complement system becomes active, there may be destruction and removal of cells and substances, which may be detrimental to the individual [32].
In the steady state, the total rate of growth, and the total rate of removal of cells from the chemostat, is D W P S ( 1 - P S / P 0 ) which is D P 0 ( 1 - D / D W ).
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IL-6 protein concentration in the pouch exudate was determined by enzyme-linked immunosorbent assay (eBioscience, San Diego, CA, USA), after removal of cells and debris by centrifugation.
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