Subsequently, the cells were incubated with CellScrub for 5 min after the removal of cell media (to remove surface-bound polyplexes), followed by trypsinization, centrifugation, and then suspension in PBS.
Crude oil mass was determined after removal of cell mass by centrifugation.
The final suspension was centrifuged at 4000 rpm for 5 min for the removal of cell debris.
After the removal of cell debris by centrifugation, the cell-free extract was incubated at 70°C for 30 min.
Removal of cell materials by alkaline treatment was considered more economically feasible by Choi and Lee (1997, 1999) as compared to an organic solvent based PHA purification process.
Phagocyte recruitment at the site of tissue injury also plays an important role for the removal of cell debris, the restitution of tissue integrity and healing.
Next to a decreased removal of cell materials, treatment at 0.2 M NH4OH showed more severe PHB degradation, which resulted in a lower recovery yield.
4′,6-Diamidino-2-phenylindole (DAPI) and Masson' trichrome staining showed complete removal of cell debris, while structure integrity of extracellular matrix (ECM) was remained.
The steps may include enzymatic reactions, tag hybridization, buffer exchange, and selective removal of cell debris and by-products of the reactions.
This process, called takeover, involves massive programmed cell death (PCD) of zooid organs via apoptosis followed by programmed removal of cell corpses by blood phagocytes within approximately 1 day.
After the termination of the fermentation period, the fermented broth was filtered through muslin cloth followed by centrifugation (Sigma 2 16 PK) for 10 min at 10,000×g and 4 °C for the removal of cell mass and unwanted particles.
