Sentence examples for removal of buffer from inspiring English sources

In the second protocol (Series #2), removal of buffer components and sample desalting were accomplished using centrifugal MACROSpin plates (Nest Group, Southborough, MA, USA).

A wash step entails the resuspension of the concentrated beads with WB, followed by removal of buffer through the use of the MPC.

CTH1 was refolded by a stepwise removal of buffer containing urea ending up in 20 mM Citrate-phosphate buffer pH 4, 10% glycerol, 1 mM cysteine which yielded soluble protein.

These steps were found to be critical for the removal of buffer from the first dimension.

The cells were washed three more times with cold HBSS followed by spin at 500  g and removal of buffer.

This resulted in better removal of buffer salts, as demonstrated by the lower scores on comp. 1 for the " plate, buffer, int.

The removal of the pretreatment solution is performed by successive additions and removals of buffer.

Following the removal of fixation buffer, cells were incubated with 0.1 M cacodylate buffer (pH7.4) at 4 oC for 10 min.

Finally, covalent coupling of the antibody to the surface of the slide allows complete removal of wash buffer without concern of removing samples during the assay.

After removal of blocking buffer, sections were incubated at 4°C overnight with fluorescein-reporter conjugated and Biotin conjugated c-Met antibodies prepared in blocking buffer.

Removal of excess buffer with tissue paper promoted enough squashing, which allowed for detailed visualization of cells and cysts at the different steps of spermatogenesis and also for checking for motility of sperm/spermatid tails.